Pdf a contagious disease characterized with severe pruritis, weight loss and alopecia was observed in laboratory mice in. Using a newly generated ribosomal rna sequence of m. The laboratory mouse is the workhorse of immunology, used as a model of mammalian immune function, but how well immune responses of laboratory mice reflect those of free living animals is. Myocoptes musculinus koch, 1836 references edit tenquist, j. Myocoptes musculinus is common, but typically asymptomatic, on laboratory mice. To ensure complete resuspension, vortex each tube thoroughly. Agents were diagnosed as myocoptes musculinus according to their morphological characteristics. Center for biomedical research iacuc standard operating. Therefore, we decided to use the fur mite at very low prevalence as a reference organism during the whole study period to ensure the validity of this method. Aspicularis tetraptera, syphacia obelata, syphacia muris d. Reptiles imported into slovenia from 2000 to 2005, specimens of native species taken from the wild and captive bred species were investigated.
This trial was conducted in order to assess the safety and efficacy of a single topical administration of eprinomectin 5mgkg bw in a naturally infested laboratory. Severe myocoptes musculinus infestation and treatment in laboratory mice article pdf available in veteriner fakultesi dergisi 571. Evaluation of diagnostic methods for myocoptes musculinus according to age and treatment status of mice mus musculus. House mice, mus musculus, are reservoirs of zoonotic pathogens and parasites, but most consumers assume mice sold by pet stores are disease and parasite free. Myocoptes musculinus is another commonly seen fur mite of mice. Isolation of the mite myocoptes musculinus koch from the spinifex hopping mouse notomys alexis. The animals remained free from mites for at least 8. The laboratory animal facility housed approximately 2700 adult. Evaluation of diagnostic methods for myocoptes musculinus. Clinical signs, pathology, and research complications, contains informative tables covering all the diseases listed in the volume, arranged to help in the diagnosis.
A revision of the annotated checklist of ectoparasites of terrestrial mammals in new zealand. Soiled bedding sentinels for the detection of fur mites in mice. For claims with a date of service on or after october 1, 2015, use an. Larvae of the genus leptotrombidium acariformes, trombiculidae are the wellknown vectors of tsutsugamushi disease agents rickettsia orientalis nagayo, tamiya, mitamura et sato, 1930 in countries of the southeast asia and on the japanese islands. Myocoptes musculinus patchy hair loss and itching radfordia affinisitching, small scabs to extensive ulceration polyplax senatapoor thrift, restlessness, itching, anemia rats radfordia ensiferasurfacedwelling fur mite causing hair loss, scaly skin, itching, poor thrift liponyssus bacotifeeds on blood of rats, lives in environment. Exotic reptiles originating from the wild can be carriers of many different pathogens and some of them can infect humans.
This component contains high copy number template and is a very significant. Prevention of infestation is through purchase of parasitefree rats, and. Detection of myocoptes musculinus in fur swab and fecal. The second section, individual disease agents and their effects on research, describes the diagnosis and control of each infectious agent, and the last section, diagnostic indexes. Production of specificmitefree rats and mice by two methods. Pdf ectoparasites of house mice mus musculus from pet. Myocoptes musculinus koch, 1836 espesye sa mananap nga kaka nga una nga gihulagway ni koch ni adtong 1836 ang myocoptes musculinus.
Mites myocoptes musculinus are the common parasites of laboratory mice in cultures. Effect of delphinium staphisagria in murine infection by. Effect of delphinium staphisagria in murine infection by myocoptes musculinus. Myocoptes musculinus wikispecies, free species directory. Pdf several colonies of mice located in our research facility were infested with mites identified as. Severe myocoptes musculinus infestation and treatment in laboratory free download as pdf file. Lesions began to heal after the first treatment with each acaricide and the g.
Myocoptes musculinus an overview sciencedirect topics. A total of 949 reptiles 55 snakes, 331 lizards and 563 turtles, belonging to 68 different species, were examined for the presence. Pdf isolation of the mite myocoptes musculinus koch from. Laboratory mice were freed from infestations by the listrophorid mite, myocoptes musculinus koch, by submerging them three times at intervals of five days in a dip containing 0. Current methods for detecting mites in mouse colonies have limitations in terms of cost, accuracy, and throughput. Acariasis in animals is caused by a variety of mites class arachnida, subclass. Effects of insecticides on acariasis in mice laboratory animals. Primerdesign ltd tm myocoptes musculinus genesig easy kit. Skin and joints infectious diseases of mice and rats the. Every effort will be made to maintain separation of species to limit crossspecies transmission of. To prevent disease, cbr procures selected, pathogen free animals.
Monogenetic trematodes fecal floation in 10% sucrose. Radfordia affinis, radfordia ensifera, myocoptes musculinus pinworms. Detection of murine astrovirus and myocoptes musculinus in. Health monitoring report for mice harvard university. Pododermatitis is relatively common in guinea pigs. The incidence of infection with fur mites is common. Myocoptes musculinus, myobia musculi and radfordia affinis, and evidence suggests that dar mice may be infested with all species. Click here to buy this book in print or download it as a free pdf, if available. Mouse fur mite fact sheet vice president for research. In very heavy infestations, the appearance of the legs can make the fur of albino animals appear brownish at. Mouse specific pathogen free spf criteria the university. A method for eradication of the mite, myocoptes musculinus.
Here we compared the efficacy of furpluck, cage pcr, and fur pcr testing of mice naturally infested with myocoptes musculinus and make recommendations. All mice were infested with 1 or more of the following ectoparasites. Ivomec administered orally and atgard completely eradicated both myocoptes musculinus and myobia musculi infestations in conventional colonies of mice. The free library general interestinformational zoology and wildlife conservation acta zoologica mexicana nueva serie january 1, 2011. Efficacy and safety of topical eprinomectin to control. The dip was prepared by dissolving the dmc in 95 per cent.
Efficacy and safety of topical eprinomectin to control myocoptes. In january 2012, mice exhibiting excessive pruritus as the only clinical sign were signaled at a laboratory animal facility of the university of turin, italy. Research complications infectious diseases of mice and. The zoonotic nature of some mites may also pose a health hazard to workers. Myocoptes musculinus primerdesign ltdtm 50 reaction genesig easy kit for use on the genesig q16 for general laboratory and research use only myocoptes musculinus 1 genesig easy kit handbook hb10. We compared all 3 diagnostic methods in groups of infested and noninfested control mice. Of the eight pairs of legs, the 3rd and 4th pairs are modified for hair clasping and heavily chitinized figure 3. We purchased 30 frozen mice from 15 pet stores in south carolina and examined them for ectoparasites. Liponyssoides sanguineus, myocoptes musculinus, myobia musculi, ornithonyssus bacoti, polyplax serrata, or. Myocoptes musculinus is the most common fur mite identified among laboratory mice. Detecting and controlling murine fur mites continues to be challenging. Fur, skin, and ear mites technical sheet charles river research models and services t.
Evaluation of five treatment regimens and five diagnostic. Ectoparasites of house mice mus musculus from pet stores. Phenomenon of simultaneous feeding of leptotrombidium. Mouse specific pathogen free spf criteria 6101 the university of chicago animal resources center the university of chicago rodent clinical services strives to provide the highest standards of rodent health care for research investigators. Mouse parasites amo at university of missouri columbia. Resuspend the primerprobe mixes in the rnasednase free water supplied, according to the table below. Pdf, epub, full text, audio unceasing customer service. Myocoptes musculinus causes lesions similar to but usually milder than those caused. Myobia musculi and radfordia affinis inhabit the hair shafts and skin surface of the intrascapular and dorsal cervical regions.
Eggs hatch in 5 days and are usually attached to the middle third of the hair shaft. To address these limitations, we developed pcr assays to detect myocoptes musculinus in fecal samples. This is the most common ectoparasite of the laboratory mouse but frequently occurs in conjunction with myobia musculi. Pdf evaluation of five treatment regimens and five diagnostic. Pagka karon wala pay siak nga nalista ubos niini niya. The effect of cysteine and b group vitamins on the skin of. Animals with inapparent mite infestations may still have sequelae that interfere with research. Rodents and rabbits make up a significant and increasing part of small animal veterinary practice. To further evaluate their efficacy in treating myocoptes spp.
Here we compared the efficacy of furpluck, cage pcr, and fur pcr testing of mice naturally infested with myocoptes musculinus and make recommendations regarding the application of these diagnostic strategies in aged or treated mice. Rodentolepis nana adults range from 25 to 40 mm long and less than l mm wide and have an armed rostellum see photo. Current treatment options for murine fur mites have limitations in safety and efficacy. A breeding colony consisting of 250 different strains of mice was treated with the topical acaricide selamectin for the mouse fur mite myocoptes musculinus, with no apparent ill effect, suggesting that this drug is safe for use in mice. Severe myocoptes musculinus infestation and treatment in laboratory mice. Diagnostic evaluation was performed, using five methods reported in literature and included skin scrape, observation, stereomicroscopic examination, scotch tape, and hair pluck. Ang myocoptes musculinus sakop sa kahenera nga myocoptes sa kabanay nga myocoptidae. Skin disease is a common reason for presentation, as it is easily noticed by the owner, even in species that are not regularly handled. Myocoptes musculinus fur mite direct exam of pelage psorergates simplex burrowing mite direct exam. Use of selamectin and moxidectin in the treatment of mouse.
In very heavy infestations, the appearance of the legs can make the fur of albino animals appear brownish at sites of mite concentration. Myocoptes musculinus is found on the hair shafts and skin surface over the flanks and rump in low numbers. The life cycle includes egg, larva, protonymph, tridonymph, and adult stages. Ectoparasites of house mice mus musculus from pet stores in south carolina, u. They include myobia musculi, radfordia affinis, myocoptes musculinus, and, less. This study evaluated whether topical lime sulfur ls is an adjunct or alternative to traditional treatment options for myocoptes musculinus. Parasites in pet reptiles acta veterinaria scandinavica. The effect of cysteine and b group vitamins on the skin of the white laboratory mouse infested with the mite myocoptes musculinus koch volume 53 issue 12 doreen p. Pdf severe myocoptes musculinus infestation and treatment in. Severe myocoptes musculinus infestation and treatment in. The comparative immunology of wild and laboratory mice.
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